Analytical Technologies in Biotechnology

Course Description

Topics included: Microscopy, Radioisotope techniques, Chromatographic methods, Electrophoresis, Centrifugation techniques, Spectroscopic Techniques, Polymerase Chain Reaction (PCR), DNA sequencing, ELISA.

Analytical Technologies in Biotechnology
Lecture 29: Types of Centrifuges and Analytical Ultracentrifugation Method
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Video Lectures & Study Materials

Visit the official course website for more study materials:

# Lecture Play Lecture
I. Microscopy
1 Basic concepts in microscopy 1 (48:17) Play Video
2 Basic concepts in microscopy 2 (43:23) Play Video
3 Dark-field and phase contrast microscopy (45:49) Play Video
4 Differential interference contrast and polarization (44:00) Play Video
5 Fluorescence and confocal microscopy (41:07) Play Video
6 Transmission electron microscopy (46:27) Play Video
7 Transmission electron microscopy cont. and scanning electron microscopy (43:31) Play Video
II. Radioisotopes Techniques
8 Basic concepts 1 (43:05) Play Video
9 Basic concepts 2 (41:47) Play Video
10 GM counting and Scintillation counting (47:09) Play Video
11 Scintillation counting continued (39:54) Play Video
12 Autoradiography and RIA (54:19) Play Video
13 Safety aspects and applications (49:45) Play Video
III. Chromatographic Methods
14 Introduction and Basic concepts in chromatography 1 (42:21) Play Video
15 Basic concepts in chromatography 2 (47:48) Play Video
16 Low-pressure liquid chromatography (LPLC) and high performance liquid chromatography (52:48) Play Video
17 Ion-exchange chromatography (48:39) Play Video
18 Gel-filtration chromatography (48:57) Play Video
19 Affinity chromatography (41:05) Play Video
20 Gas-liquid chromatography (50:43) Play Video
IV. Electrophoresis
21 Basic concepts in electrophoresis (47:25) Play Video
22 Horizontal and vertical gel electrophoresis (46:03) Play Video
23 Native gel electrophoresis and SDS-PAGE (47:52) Play Video
24 Isoelectric focusing (IEF), 2-D gel electrophoresis and protein detection methods (52:50) Play Video
25 Electrophoresis of nucleic acids (50:41) Play Video
26 Immunoelectrophoresis and capillary electrophoresis (54:08) Play Video
V. Centrifugation Techniques
27 Introduction and basic concepts 1 (46:17) Play Video
28 Basic concepts-2 (48:17) Play Video
29 Types of centrifuges and analytical ultracentrifugation method (55:03) Play Video
30 Separation methods in preparative ultracentrifuges (51:14) Play Video
31 Types of rotors (48:16) Play Video
32 Types of rotors cont. and care of rotors (47:58) Play Video
VI. Spectroscopic Techniques
33 Introduction and basic concepts (50:57) Play Video
34 UV-Visible spectroscopy (49:58) Play Video
35 Infrared and fluorescence spectroscopy (54:43) Play Video
36 Circular dichroism (CD) spectroscopy (46:55) Play Video
37 Nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography (1:04:26) Play Video
38 Atomic spectroscopy and mass spectrometry (51:22) Play Video
VII. Polymerase Chain Reaction
39 Polymerase chain reaction(PCR) (52:20) Play Video
40 DNA sequencing methods (1:00:45) Play Video
41 Enzyme linked immunosorbent assay (ELISA) (44:13) Play Video


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