Getting Started in Cryo-EM

Course Description

This class covers the fundamental principles underlying cryo-electron microscopy starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. See the course outline for a full list of topics.

Lectures by Grant Jensen, Professor of Biology, Caltech; Investigator, Howard Hughes Medical Institute. Recorded at Caltech in 2013 -14.
Learn more about the Jensen Lab at Caltech:

Getting Started in Cryo-EM
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Video Lectures & Study Materials

Visit the official course website for more study materials:

# Lecture Play Lecture
1 Getting Started in Cryo-EM with Professor Grant Jensen Play Video
2 Welcome to Cryo-EM Play Video
3 Introduction: Why Electrons? Play Video
4 Part 1: Basic Anatomy of the EM Play Video
5 Part 1: Electron Guns Play Video
6 Part 1: EM Lenses Play Video
7 Part 1: The Column Play Video
8 Part 1: Sample Chamber Play Video
9 Part 1: Energy Filters Play Video
10 Part 1: Detectors Play Video
11 Part 1: Vacuum Systems Play Video
12 Part 1: Summary and Safety Play Video
13 Part 2: Fourier Transforms for Beginners Play Video
14 Part 2: 1-D Sine Waves and Their Sums Play Video
15 Part 2: 1-D Reciprocal Space Play Video
16 Part 2: 2-D Waves and Images Play Video
17 Part 2: 2-D Transforms and Filters Play Video
18 Part 2: 3-D Waves and Transforms Play Video
19 Part 2: Convolution and Cross-Correlation Play Video
20 Part 3: Image Formation Play Video
21 Part 3: Amplitude and Phase Contrast 2016 Play Video
22 Part 3: Wave Propagation and Phase Shifts Play Video
23 Part 3: The Contrast Transfer Function Play Video
24 Part 3: Defocus and Its Effects Play Video
25 Part 3: Envelopes Play Video
26 Part 3: CTF Correction Play Video
27 Part 4: Fundamental Challenges in Biological TEM Play Video
28 Part 4: Sample Prep: Room Temperature Methods Play Video
29 Part 4: Sample Prep: Methods Involving Freezing Play Video
30 Part 4: Sample Prep: Grids Play Video
31 Part 4: 3-D Reconstruction Play Video
32 Part 4: Dose Limitations Play Video
33 Part 5: Tomography Play Video
34 Part 5: Tomography Data Collection and Reconstruction Play Video
35 Part 5: Tomography Identfying Objects of Interest Play Video
36 Part 5: Tomography Limitations Play Video
37 Part 5: Tomography Parameters and Handedness Play Video
38 Part 6: Single Particle Analysis Play Video
39 Part 6: SPA Sample Prep Play Video
40 Part 6: SPA Data Collection Play Video
41 Part 6: SPA Reconstruction Basic Workflow Play Video
42 Part 6: SPA Reconstruction Additional Topics Play Video
43 Part 6: SPA Interpretations and Limitations Play Video
44 Part 7: 2D Crystallography Intro and Sample Prep Play Video
45 Part 7: FT of a 2D Crystal Play Video
46 Part 7: 2D Crystallography Data Collection and Reconstruction Play Video
47 Part 7: Helical Tubes Play Video
48 Part 7: Farewell Play Video


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