Getting Started in Cryo-EM

Video Lectures

Displaying all 48 video lectures.
Lecture 1
Getting Started in Cryo-EM with Professor Grant Jensen
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Getting Started in Cryo-EM with Professor Grant Jensen
Visit the course website: http://cryo-em-course.caltech.edu
Learn more about the Jensen Lab: http://www.jensenlab.caltech.edu

Produced in association with Caltech Academic Media Technologies. ©2015 California Institute of Technology
Lecture 2
Welcome to Cryo-EM
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Welcome to Cryo-EM
Lecture 3
Introduction: Why Electrons?
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Introduction: Why Electrons?
Lecture 4
Part 1: Basic Anatomy of the EM
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Part 1: Basic Anatomy of the EM
Lecture 5
Part 1: Electron Guns
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Part 1: Electron Guns
Lecture 6
Part 1: EM Lenses
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Part 1: EM Lenses
Lecture 7
Part 1: The Column
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Part 1: The Column
Lecture 8
Part 1: Sample Chamber
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Part 1: Sample Chamber
Lecture 9
Part 1: Energy Filters
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Part 1: Energy Filters
Lecture 10
Part 1: Detectors
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Part 1: Detectors
Lecture 11
Part 1: Vacuum Systems
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Part 1: Vacuum Systems
Lecture 12
Part 1: Summary and Safety
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Part 1: Summary and Safety
Lecture 13
Part 2: Fourier Transforms for Beginners
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Part 2: Fourier Transforms for Beginners
Lecture 14
Part 2: 1-D Sine Waves and Their Sums
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Part 2: 1-D Sine Waves and Their Sums
Lecture 15
Part 2: 1-D Reciprocal Space
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Part 2: 1-D Reciprocal Space
Lecture 16
Part 2: 2-D Waves and Images
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Part 2: 2-D Waves and Images
Lecture 17
Part 2: 2-D Transforms and Filters
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Part 2: 2-D Transforms and Filters
Lecture 18
Part 2: 3-D Waves and Transforms
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Part 2: 3-D Waves and Transforms
Lecture 19
Part 2: Convolution and Cross-Correlation
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Part 2: Convolution and Cross-Correlation
Lecture 20
Part 3: Image Formation
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Part 3: Image Formation
Lecture 21
Part 3: Amplitude and Phase Contrast 2016
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Part 3: Amplitude and Phase Contrast 2016
Lecture 22
Part 3: Wave Propagation and Phase Shifts
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Part 3: Wave Propagation and Phase Shifts
Lecture 23
Part 3: The Contrast Transfer Function
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Part 3: The Contrast Transfer Function
Lecture 24
Part 3: Defocus and Its Effects
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Part 3: Defocus and Its Effects
Lecture 25
Part 3: Envelopes
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Part 3: Envelopes
Lecture 26
Part 3: CTF Correction
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Part 3: CTF Correction
Lecture 27
Part 4: Fundamental Challenges in Biological TEM
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Part 4: Fundamental Challenges in Biological TEM
Lecture 28
Part 4: Sample Prep: Room Temperature Methods
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Part 4: Sample Prep: Room Temperature Methods
Lecture 29
Part 4: Sample Prep: Methods Involving Freezing
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Part 4: Sample Prep: Methods Involving Freezing
Lecture 30
Part 4: Sample Prep: Grids
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Part 4: Sample Prep: Grids
Lecture 31
Part 4: 3-D Reconstruction
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Part 4: 3-D Reconstruction
Lecture 32
Part 4: Dose Limitations
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Part 4: Dose Limitations
Lecture 33
Part 5: Tomography
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Part 5: Tomography
Lecture 34
Part 5: Tomography Data Collection and Reconstruction
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Part 5: Tomography Data Collection and Reconstruction
Lecture 35
Part 5: Tomography Identfying Objects of Interest
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Part 5: Tomography Identfying Objects of Interest
Lecture 36
Part 5: Tomography Limitations
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Part 5: Tomography Limitations
Lecture 37
Part 5: Tomography Parameters and Handedness
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Part 5: Tomography Parameters and Handedness
Lecture 38
Part 6: Single Particle Analysis
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Part 6: Single Particle Analysis
Lecture 39
Part 6: SPA Sample Prep
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Part 6: SPA Sample Prep
Lecture 40
Part 6: SPA Data Collection
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Part 6: SPA Data Collection
Lecture 41
Part 6: SPA Reconstruction Basic Workflow
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Part 6: SPA Reconstruction Basic Workflow
Lecture 42
Part 6: SPA Reconstruction Additional Topics
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Part 6: SPA Reconstruction Additional Topics
Lecture 43
Part 6: SPA Interpretations and Limitations
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Part 6: SPA Interpretations and Limitations
Lecture 44
Part 7: 2D Crystallography Intro and Sample Prep
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Part 7: 2D Crystallography Intro and Sample Prep
Lecture 45
Part 7: FT of a 2D Crystal
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Part 7: FT of a 2D Crystal
Lecture 46
Part 7: 2D Crystallography Data Collection and Reconstruction
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Part 7: 2D Crystallography Data Collection and Reconstruction
Lecture 47
Part 7: Helical Tubes
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Part 7: Helical Tubes
Lecture 48
Part 7: Farewell
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Part 7: Farewell