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Course

Subject:	Biology
Topic:	Cryo-electron microscopy
Views:	17,503

Educator

Name:	California Institute of Technology (Caltech)
Type:	University

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Getting Started in Cryo-EM

Video Lectures

Displaying all 48 video lectures.

Lecture 1 Play Video	Getting Started in Cryo-EM with Professor Grant Jensen Visit the course website: http://cryo-em-course.caltech.edu Learn more about the Jensen Lab: http://www.jensenlab.caltech.edu Produced in association with Caltech Academic Media Technologies. ©2015 California Institute of Technology
Lecture 2 Play Video	Welcome to Cryo-EM
Lecture 3 Play Video	Introduction: Why Electrons?
Lecture 4 Play Video	Part 1: Basic Anatomy of the EM
Lecture 5 Play Video	Part 1: Electron Guns
Lecture 6 Play Video	Part 1: EM Lenses
Lecture 7 Play Video	Part 1: The Column
Lecture 8 Play Video	Part 1: Sample Chamber
Lecture 9 Play Video	Part 1: Energy Filters
Lecture 10 Play Video	Part 1: Detectors
Lecture 11 Play Video	Part 1: Vacuum Systems
Lecture 12 Play Video	Part 1: Summary and Safety
Lecture 13 Play Video	Part 2: Fourier Transforms for Beginners
Lecture 14 Play Video	Part 2: 1-D Sine Waves and Their Sums
Lecture 15 Play Video	Part 2: 1-D Reciprocal Space
Lecture 16 Play Video	Part 2: 2-D Waves and Images
Lecture 17 Play Video	Part 2: 2-D Transforms and Filters
Lecture 18 Play Video	Part 2: 3-D Waves and Transforms
Lecture 19 Play Video	Part 2: Convolution and Cross-Correlation
Lecture 20 Play Video	Part 3: Image Formation
Lecture 21 Play Video	Part 3: Amplitude and Phase Contrast 2016
Lecture 22 Play Video	Part 3: Wave Propagation and Phase Shifts
Lecture 23 Play Video	Part 3: The Contrast Transfer Function
Lecture 24 Play Video	Part 3: Defocus and Its Effects
Lecture 25 Play Video	Part 3: Envelopes
Lecture 26 Play Video	Part 3: CTF Correction
Lecture 27 Play Video	Part 4: Fundamental Challenges in Biological TEM
Lecture 28 Play Video	Part 4: Sample Prep: Room Temperature Methods
Lecture 29 Play Video	Part 4: Sample Prep: Methods Involving Freezing
Lecture 30 Play Video	Part 4: Sample Prep: Grids
Lecture 31 Play Video	Part 4: 3-D Reconstruction
Lecture 32 Play Video	Part 4: Dose Limitations
Lecture 33 Play Video	Part 5: Tomography
Lecture 34 Play Video	Part 5: Tomography Data Collection and Reconstruction
Lecture 35 Play Video	Part 5: Tomography Identfying Objects of Interest
Lecture 36 Play Video	Part 5: Tomography Limitations
Lecture 37 Play Video	Part 5: Tomography Parameters and Handedness
Lecture 38 Play Video	Part 6: Single Particle Analysis
Lecture 39 Play Video	Part 6: SPA Sample Prep
Lecture 40 Play Video	Part 6: SPA Data Collection
Lecture 41 Play Video	Part 6: SPA Reconstruction Basic Workflow
Lecture 42 Play Video	Part 6: SPA Reconstruction Additional Topics
Lecture 43 Play Video	Part 6: SPA Interpretations and Limitations
Lecture 44 Play Video	Part 7: 2D Crystallography Intro and Sample Prep
Lecture 45 Play Video	Part 7: FT of a 2D Crystal
Lecture 46 Play Video	Part 7: 2D Crystallography Data Collection and Reconstruction
Lecture 47 Play Video	Part 7: Helical Tubes
Lecture 48 Play Video	Part 7: Farewell