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Biology
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Course
Subject:
Biology
Topic:
Cryo-electron microscopy
Views:
33,435
Educator
Name:
California Institute of Technology (Caltech)
Type:
University
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Getting Started in Cryo-EM
Video Lectures
Displaying all 48 video lectures.
Lecture 1
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Getting Started in Cryo-EM with Professor Grant Jensen
Visit the course website:
http://cryo-em-course.caltech.edu
Learn more about the Jensen Lab:
http://www.jensenlab.caltech.edu
Produced in association with Caltech Academic Media Technologies. ©2015 California Institute of Technology
Lecture 2
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Welcome to Cryo-EM
Lecture 3
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Introduction: Why Electrons?
Lecture 4
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Part 1: Basic Anatomy of the EM
Lecture 5
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Part 1: Electron Guns
Lecture 6
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Part 1: EM Lenses
Lecture 7
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Part 1: The Column
Lecture 8
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Part 1: Sample Chamber
Lecture 9
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Part 1: Energy Filters
Lecture 10
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Part 1: Detectors
Lecture 11
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Part 1: Vacuum Systems
Lecture 12
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Part 1: Summary and Safety
Lecture 13
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Part 2: Fourier Transforms for Beginners
Lecture 14
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Part 2: 1-D Sine Waves and Their Sums
Lecture 15
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Part 2: 1-D Reciprocal Space
Lecture 16
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Part 2: 2-D Waves and Images
Lecture 17
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Part 2: 2-D Transforms and Filters
Lecture 18
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Part 2: 3-D Waves and Transforms
Lecture 19
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Part 2: Convolution and Cross-Correlation
Lecture 20
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Part 3: Image Formation
Lecture 21
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Part 3: Amplitude and Phase Contrast 2016
Lecture 22
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Part 3: Wave Propagation and Phase Shifts
Lecture 23
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Part 3: The Contrast Transfer Function
Lecture 24
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Part 3: Defocus and Its Effects
Lecture 25
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Part 3: Envelopes
Lecture 26
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Part 3: CTF Correction
Lecture 27
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Part 4: Fundamental Challenges in Biological TEM
Lecture 28
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Part 4: Sample Prep: Room Temperature Methods
Lecture 29
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Part 4: Sample Prep: Methods Involving Freezing
Lecture 30
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Part 4: Sample Prep: Grids
Lecture 31
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Part 4: 3-D Reconstruction
Lecture 32
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Part 4: Dose Limitations
Lecture 33
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Part 5: Tomography
Lecture 34
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Part 5: Tomography Data Collection and Reconstruction
Lecture 35
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Part 5: Tomography Identfying Objects of Interest
Lecture 36
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Part 5: Tomography Limitations
Lecture 37
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Part 5: Tomography Parameters and Handedness
Lecture 38
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Part 6: Single Particle Analysis
Lecture 39
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Part 6: SPA Sample Prep
Lecture 40
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Part 6: SPA Data Collection
Lecture 41
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Part 6: SPA Reconstruction Basic Workflow
Lecture 42
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Part 6: SPA Reconstruction Additional Topics
Lecture 43
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Part 6: SPA Interpretations and Limitations
Lecture 44
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Part 7: 2D Crystallography Intro and Sample Prep
Lecture 45
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Part 7: FT of a 2D Crystal
Lecture 46
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Part 7: 2D Crystallography Data Collection and Reconstruction
Lecture 47
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Part 7: Helical Tubes
Lecture 48
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Part 7: Farewell
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Subject: Biology
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Getting Started in Cryo-EM