Professor Saltzman continues his presentation on DNA technology by discussing control of gene expression using two methods of RNA silencing: anti-sense therapy and RNA interference. Molecular cloning techniques to mass-produce proteins using plasmid, restriction enzymes, ligase, and antibiotic selection in bacteria are discussed. Steps and molecules involved in polymerase chain reaction are also described. Professor Saltzman explains how to detect mutations in genomic DNA, such as in sickle cell anemia patients, by gel electrophoresis and Southern blotting. Finally, he gives an example of inducing and controlling insulin expression in large animals by cloning into their genome the insulin gene with the lac promoter.

1. What Is Biomedical Engineering?
2. What Is Biomedical Engineering? (cont.)
3. Genetic Engineering
4. Genetic Engineering (cont.)
5. Cell Culture Engineering
6. Cell Culture Engineering (cont.)
7. Cell Communication and Immunology
8. Cell Communication and Immunology (cont.)
9. Biomolecular Engineering: Engineering of Immunity
10. Biomolecular Engineering: Engineering of Immunity (cont.)
11. Biomolecular Engineering: General Concepts
12. Biomolecular Engineering: General Concepts (cont.)
13. Cardiovascular Physiology
14. Cardiovascular Physiology (cont.)
15. Cardiovascular Physiology (cont.)
16. Renal Physiology
17. Renal Physiology (cont.)
18. Biomechanics and Orthopedics
19. Biomechanics and Orthopedics (cont.)
20. Bioimaging
21. Bioimaging (cont.)
22. Tissue Engineering
23. Tissue Engineering (cont.)
24. Biomedical Engineers and Cancer
25. Biomedical Engineers and Artificial Organs

The course covers basic concepts of biomedical engineering and their connection with the spectrum of human activity. It serves as an introduction to the fundamental science and engineering on which biomedical engineering is based. Case studies of drugs and medical products illustrate the product development-product testing cycle, patent protection, and FDA approval. It is designed for science and non-science majors.