Genetic Engineering (cont.) 
Genetic Engineering (cont.)
by Yale / W. Mark Saltzman
Video Lecture 4 of 25
1 rating
Views: 1,554
Date Added: March 24, 2009

Lecture Description

Professor Saltzman continues his presentation on DNA technology by discussing control of gene expression using two methods of RNA silencing: anti-sense therapy and RNA interference. Molecular cloning techniques to mass-produce proteins using plasmid, restriction enzymes, ligase, and antibiotic selection in bacteria are discussed. Steps and molecules involved in polymerase chain reaction are also described. Professor Saltzman explains how to detect mutations in genomic DNA, such as in sickle cell anemia patients, by gel electrophoresis and Southern blotting. Finally, he gives an example of inducing and controlling insulin expression in large animals by cloning into their genome the insulin gene with the lac promoter.

Course Index

Course Description


The course covers basic concepts of biomedical engineering and their connection with the spectrum of human activity. It serves as an introduction to the fundamental science and engineering on which biomedical engineering is based. Case studies of drugs and medical products illustrate the product development-product testing cycle, patent protection, and FDA approval. It is designed for science and non-science majors.

Comments

There are no comments. Be the first to post one.
  Post comment as a guest user.
Click to login or register:
Your name:
Your email:
(will not appear)
Your comment:
(max. 1000 characters)
Are you human? (Sorry)