Lecture Description
Visit the course website: cryo-em-course.caltech.edu
Learn more about the Jensen Lab: www.jensenlab.caltech.edu
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Course Index
- Getting Started in Cryo-EM with Professor Grant Jensen
- Welcome to Cryo-EM
- Introduction: Why Electrons?
- Part 1: Basic Anatomy of the EM
- Part 1: Electron Guns
- Part 1: EM Lenses
- Part 1: The Column
- Part 1: Sample Chamber
- Part 1: Energy Filters
- Part 1: Detectors
- Part 1: Vacuum Systems
- Part 1: Summary and Safety
- Part 2: Fourier Transforms for Beginners
- Part 2: 1-D Sine Waves and Their Sums
- Part 2: 1-D Reciprocal Space
- Part 2: 2-D Waves and Images
- Part 2: 2-D Transforms and Filters
- Part 2: 3-D Waves and Transforms
- Part 2: Convolution and Cross-Correlation
- Part 3: Image Formation
- Part 3: Amplitude and Phase Contrast 2016
- Part 3: Wave Propagation and Phase Shifts
- Part 3: The Contrast Transfer Function
- Part 3: Defocus and Its Effects
- Part 3: Envelopes
- Part 3: CTF Correction
- Part 4: Fundamental Challenges in Biological TEM
- Part 4: Sample Prep: Room Temperature Methods
- Part 4: Sample Prep: Methods Involving Freezing
- Part 4: Sample Prep: Grids
- Part 4: 3-D Reconstruction
- Part 4: Dose Limitations
- Part 5: Tomography
- Part 5: Tomography Data Collection and Reconstruction
- Part 5: Tomography Identfying Objects of Interest
- Part 5: Tomography Limitations
- Part 5: Tomography Parameters and Handedness
- Part 6: Single Particle Analysis
- Part 6: SPA Sample Prep
- Part 6: SPA Data Collection
- Part 6: SPA Reconstruction Basic Workflow
- Part 6: SPA Reconstruction Additional Topics
- Part 6: SPA Interpretations and Limitations
- Part 7: 2D Crystallography Intro and Sample Prep
- Part 7: FT of a 2D Crystal
- Part 7: 2D Crystallography Data Collection and Reconstruction
- Part 7: Helical Tubes
- Part 7: Farewell
Course Description
This class covers the fundamental principles underlying cryo-electron microscopy starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. See the course outline for a full list of topics.
Lectures by Grant Jensen, Professor of Biology, Caltech; Investigator, Howard Hughes Medical Institute. Recorded at Caltech in 2013 -14.
Learn more about the Jensen Lab at Caltech: http://www.jensenlab.caltech.edu