Visit the course website: cryo-em-course.caltech.edu
Learn more about the Jensen Lab: www.jensenlab.caltech.edu

Produced in association with Caltech Academic Media Technologies. ©2015 California Institute of Technology

1. Getting Started in Cryo-EM with Professor Grant Jensen
2. Welcome to Cryo-EM
3. Introduction: Why Electrons?
4. Part 1: Basic Anatomy of the EM
5. Part 1: Electron Guns
6. Part 1: EM Lenses
7. Part 1: The Column
8. Part 1: Sample Chamber
9. Part 1: Energy Filters
10. Part 1: Detectors
11. Part 1: Vacuum Systems
12. Part 1: Summary and Safety
13. Part 2: Fourier Transforms for Beginners
14. Part 2: 1-D Sine Waves and Their Sums
15. Part 2: 1-D Reciprocal Space
16. Part 2: 2-D Waves and Images
17. Part 2: 2-D Transforms and Filters
18. Part 2: 3-D Waves and Transforms
19. Part 2: Convolution and Cross-Correlation
20. Part 3: Image Formation
21. Part 3: Amplitude and Phase Contrast 2016
22. Part 3: Wave Propagation and Phase Shifts
23. Part 3: The Contrast Transfer Function
24. Part 3: Defocus and Its Effects
25. Part 3: Envelopes
26. Part 3: CTF Correction
27. Part 4: Fundamental Challenges in Biological TEM
28. Part 4: Sample Prep: Room Temperature Methods
29. Part 4: Sample Prep: Methods Involving Freezing
30. Part 4: Sample Prep: Grids
31. Part 4: 3-D Reconstruction
32. Part 4: Dose Limitations
33. Part 5: Tomography
34. Part 5: Tomography Data Collection and Reconstruction
35. Part 5: Tomography Identfying Objects of Interest
36. Part 5: Tomography Limitations
37. Part 5: Tomography Parameters and Handedness
38. Part 6: Single Particle Analysis
39. Part 6: SPA Sample Prep
40. Part 6: SPA Data Collection
41. Part 6: SPA Reconstruction Basic Workflow
42. Part 6: SPA Reconstruction Additional Topics
43. Part 6: SPA Interpretations and Limitations
44. Part 7: 2D Crystallography Intro and Sample Prep
45. Part 7: FT of a 2D Crystal
46. Part 7: 2D Crystallography Data Collection and Reconstruction
47. Part 7: Helical Tubes
48. Part 7: Farewell

This class covers the fundamental principles underlying cryo-electron microscopy starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. See the course outline for a full list of topics.

Lectures by Grant Jensen, Professor of Biology, Caltech; Investigator, Howard Hughes Medical Institute. Recorded at Caltech in 2013 -14.
Learn more about the Jensen Lab at Caltech: http://www.jensenlab.caltech.edu