This class covers the fundamental principles underlying cryo-electron microscopy starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. See the course outline for a full list of topics.
Lectures by Grant Jensen, Professor of Biology, Caltech; Investigator, Howard Hughes Medical Institute. Recorded at Caltech in 2013 -14.
Learn more about the Jensen Lab at Caltech: http://www.jensenlab.caltech.edu