Part 7: Farewell 
Part 7: Farewell
by Caltech
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Date Added: May 13, 2017

Lecture Description

This video lecture, part of the series Getting Started in Cryo-EM by Prof. , does not currently have a detailed description and video lecture title. If you have watched this lecture and know what it is about, particularly what Biology topics are discussed, please help us by commenting on this video with your suggested description and title. Many thanks from,

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Course Index

  1. Getting Started in Cryo-EM with Professor Grant Jensen
  2. Welcome to Cryo-EM
  3. Introduction: Why Electrons?
  4. Part 1: Basic Anatomy of the EM
  5. Part 1: Electron Guns
  6. Part 1: EM Lenses
  7. Part 1: The Column
  8. Part 1: Sample Chamber
  9. Part 1: Energy Filters
  10. Part 1: Detectors
  11. Part 1: Vacuum Systems
  12. Part 1: Summary and Safety
  13. Part 2: Fourier Transforms for Beginners
  14. Part 2: 1-D Sine Waves and Their Sums
  15. Part 2: 1-D Reciprocal Space
  16. Part 2: 2-D Waves and Images
  17. Part 2: 2-D Transforms and Filters
  18. Part 2: 3-D Waves and Transforms
  19. Part 2: Convolution and Cross-Correlation
  20. Part 3: Image Formation
  21. Part 3: Amplitude and Phase Contrast 2016
  22. Part 3: Wave Propagation and Phase Shifts
  23. Part 3: The Contrast Transfer Function
  24. Part 3: Defocus and Its Effects
  25. Part 3: Envelopes
  26. Part 3: CTF Correction
  27. Part 4: Fundamental Challenges in Biological TEM
  28. Part 4: Sample Prep: Room Temperature Methods
  29. Part 4: Sample Prep: Methods Involving Freezing
  30. Part 4: Sample Prep: Grids
  31. Part 4: 3-D Reconstruction
  32. Part 4: Dose Limitations
  33. Part 5: Tomography
  34. Part 5: Tomography Data Collection and Reconstruction
  35. Part 5: Tomography Identfying Objects of Interest
  36. Part 5: Tomography Limitations
  37. Part 5: Tomography Parameters and Handedness
  38. Part 6: Single Particle Analysis
  39. Part 6: SPA Sample Prep
  40. Part 6: SPA Data Collection
  41. Part 6: SPA Reconstruction Basic Workflow
  42. Part 6: SPA Reconstruction Additional Topics
  43. Part 6: SPA Interpretations and Limitations
  44. Part 7: 2D Crystallography Intro and Sample Prep
  45. Part 7: FT of a 2D Crystal
  46. Part 7: 2D Crystallography Data Collection and Reconstruction
  47. Part 7: Helical Tubes
  48. Part 7: Farewell

Course Description

This class covers the fundamental principles underlying cryo-electron microscopy starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. See the course outline for a full list of topics.

Lectures by Grant Jensen, Professor of Biology, Caltech; Investigator, Howard Hughes Medical Institute. Recorded at Caltech in 2013 -14.
Learn more about the Jensen Lab at Caltech: http://www.jensenlab.caltech.edu

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